Preferential PCR amplification of alleles: mechanisms and solutions.
نویسندگان
چکیده
منابع مشابه
Preferential Amplification of Pathogenic Sequences
The application of next generation sequencing (NGS) technology in the diagnosis of human pathogens is hindered by the fact that pathogenic sequences, especially viral, are often scarce in human clinical specimens. This known disproportion leads to the requirement of subsequent deep sequencing and extensive bioinformatics analysis. Here we report a method we called "Preferential Amplification of...
متن کاملStrategy for controlling preferential amplification and avoiding false negatives in PCR typing.
The use of the PCR method for routine testing has increased dramatically during recent years. Most assays involve co-amplification either of an internal control, of several alleles at a given locus or of a variety of bands produced by low-stringency primer annealing. In such multiplex reactions, certain products will often amplify preferentially. Amplimers that are more sensitive can be outcomp...
متن کاملAddition of a competitive primer can dramatically improve the specificity of PCR amplification of specific alleles.
Genet. 3:103-112. 16.Pepin, L., Y. Amigues, A. Lepingle, J.L. Berthier, A. Bensaid and D. Vaiman. 1995. Sequence conservation of microsatellites between Bos taurus (cattle), Capra hircus (goat) and related species. Examples of use in parentage testing and phylogeny analysis. Heredity. Pt 1 74:53-61. 17.Primmer, C.R. and M.E. Matthews. 1993. Canine tetranucleotide repeat polymorphism at the VIAS...
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Alpha 1 -anlitrypsi11 alleles in patients with pulmonary emphysema, detected by DNA amplification (PCR) and oligonucleotide probes. K. Bruun-Petersen, G. BruwaPetersen, R. Daltl, B. Larsen, S. K~lvraa, J. Koch, L. Bolund, N. Gregersen. ABSTRACT: Alpha 1 -antltrypsln (AAT) deficiency Is a serious predisposing factor for the development of pulmonary emphysema. • Dept of Respiratory Diseases, Univ...
متن کاملImproved PCR amplification/Hhal restriction for unambiguous determination of apolipoprotein E alleles.
We present a modification to the polymerase chain reaction amplification/Hhal restriction isotyping method for human apolipoprotein (apo) E. This method includes a mutagenic forward primer and 5'-end labeling of both primers. These modifications of the original method described by Hixon and Vernier (J Lipid Res 1990;31:545-8) allow sensitive and unambiguous determination of apoE genotypes.
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ژورنال
عنوان ژورنال: Genome Research
سال: 1992
ISSN: 1088-9051
DOI: 10.1101/gr.1.4.241